The plasma levels of histamine (A) and IgE (B) in D. farinae extract-sensitized Nc/Nga mice. AC (10 mg/mouse/day) and Protopic ointment (50 mg/mouse/day) were topically applied on the back and ears once daily for 4 weeks. The concentrations of histamine and IgE were measured by ELISA (mean ± SEM, n = 5), ##: P < 0. 01 compared with the normal group, *: P < 0. 05 compared with the D. farinae-induced control group.