The effect of YQFS on the expression of ERK and p-ERK in HCT-116 cells. Western blotting assays were carried out to detect the activation of ERK in HCT-116 cells. HCT-116 cells were treated with YQFS at 8 mg/ml, 16 mg/ml and 32 mg/ml for 24 h respectively. Western blotting with was performed with an anti-β-actin antibody to ensure equal loading of proteins in each lane. The bolts were photographed and quantitated for each sample and the data are from three independent experiments.