|
Fraction
|
Weight (mg)
|
P. falciparum K1 IC50(μg/ml)
|
Cytotoxicity CC50(μg/ml)
|
SI
|
IC50fr. IC50CQ
|
IC50fr. IC50Art
|
CC50fr. CC50Pdx
|
|---|
|
AKLM 2
|
2,500
|
0.90 ± 0.11
|
21.0 ± 3.87
|
23.3
|
14
|
450
|
3,500
|
|
AKLM 2.1
|
15.3
|
5.0 ± 1.31
|
>90
|
18.0
|
78
|
2,500
|
>15,000
|
|
AKLM 2.2
|
26.9
|
1.09 ± 0.26
|
79.1 ± 7.60
|
72.6
|
17
|
545
|
13,183
|
|
AKLM 2.3
|
57.6
|
4.11 ± 0.29
|
53.1 ± 9.20
|
12.9
|
64
|
2,055
|
8,850
|
|
AKLM 2.4
|
98.7
|
1.16 ± 0.18
|
13.8 ± 1.80
|
11.9
|
18
|
580
|
2,300
|
|
AKLM 2.5
|
126.1
|
3.63 ± 0.04
|
24.6 ± 3.30
|
6.8
|
57
|
1,815
|
4,100
|
|
AKLM 2.6
|
115.7
|
1.23 ± 0.31
|
39.5 ± 2.20
|
32.1
|
19
|
615
|
6,583
|
|
AKLM 2.7
|
239.0
|
3.41 ± 0.48
|
85.4 ± 4.60
|
25.0
|
53
|
1,705
|
14,233
|
|
AKLM 2.8
|
478.3
|
5.0 ± 0.53
|
>90
|
18.0
|
78
|
2,500
|
>15,000
|
|
AKLM 2.9
|
351.8
|
2.40 ± 0.57
|
56.7 ± 8.00
|
23.6
|
38
|
1,200
|
9,450
|
|
AKLM 2.10
|
301.9
|
0.89 ± 0.20
|
35.3 ± 5.31
|
39.7
|
14
|
445
|
5,883
|
|
AKLM 2.11
|
672.8
|
0.64 ± 0.34
|
44.7 ± 4.45
|
69.8
|
10
|
320
|
7,450
|
-
HSCCC High speed counter current chromatography; AKLM Annickia kummeriae leaf methanol extract, P. falciparum K1 used for anti-plasmodial assays, rat myoblast L-6 cells used for cytotoxicity assays, CQ chloroquine (IC50 0.063 ± 0.034 μg/ml), Art - artemisinin (IC50 0.002 ± 0.00001 μg/ml), Pdx - podophyllotoxin (IC50 0.009 ± 0.003 μg/ml).
