Table 1 Primer sequences and PCR conditions
Gene name | Tm (°C) | Size (bp) | Sequence 5′-3′ |
|---|---|---|---|
Sterol regulatory element binding transcription factor 2 | 55 | 177 | F: GGCCTCTCCTTTAACCCCTT |
R: CACCATTTACCAGCCACAGG | |||
(SREBP2) | |||
Liver X receptor | 55 | 119 | F: TCCTACACGAGGATCAAGCG |
(LXR) | R: AGTCGCAATGCAAAGACCTG | ||
Low density lipoprotein receptor | 55 | 173 | F: GCGTATCTGTGGCTGACACC |
(LDLR) | R: TGTCCACACCATTCAAACCC | ||
3-hydroxy-3methylglutary-CoA | 55 | 151 | F: GTGGCAGAAAGAGGGAAAGG |
R: CGCCTTTGTTTTCTGGTTGA | |||
(HMG-CoA) | |||
Tumor necrosis factor alpha | 55 | 275 | F: AACATCCAACCTTCCCAAACG |
(TNFα) | R: GACCCTAAGCCCCCAATTCTC | ||
Interleukin 6 | 55 | 162 | F: TTGCCTTCTTGGGACTGATG |
(IL-6) | R: CCACGATTTCCCAGAGAACA | ||
Intercelluar adhesion molecule 1 | 55 | 176 | F: GTGGGTCGAAGGTGGTTCTT |
(ICAM1) | R: GCAGTTCCAGGGTCTGGTTT | ||
Vascular cell adhesion molecule 1 | 55 | 163 | F: CTCAGGTGGCTGCACAAGTT |
(VCAM1) | R: AGAGCTCAACACAAGCGTGG | ||
Transforming growth factor beta1 | 55 | 169 | F: GCGGCAGCTGTACATTGACT |
(TNFβ) | R: CCGGGTTGTGTTGGTTGTAG | ||
Fibronectin 1 | 55 | 197 | F: AGCTTTTCCCTGCACCTGAT |
R: GGTGGGTGTCACCTGACTGA |