Figure 1

Apoptosis induction in HeLa cells after treatment with marine bacterial extracts. Detection of externalization of PS from cell membrane was done using APOPercentage assay. Cells were stained with dye and fluorescence was measured using flow cytometry. The photographs were taken by using inverted microscope. Apoptosis is shown as the percentage of cells that uptake APOPercentage dye relative to untreated cells. A) Cells were treated with H₂O₂ or extracts at a final concentration of 500 μg/mL for 48 h, and B) for 24 h. The Z- factor was calculated to be 0.68 and 0.77 for 48 h and 24 h experiments, respectively. C) The morphology of cancer cells after 24 h of treatment with chosen extracts.