Effects of AEtLP on histological parameters and mucin secretion in Lop-induced constipated rats. (A) H&E-stained sections of transverse colons rats from the No-treated group (a and b), AEtLP-treated group (c and d), Lop + vehicle-treated group (e and f), or Lop + AEtLP-treated group (g and h) were observed at two different magnifications using a light microscope. (B) Villus length, crypt layer thickness, and muscle thickness are presented as graphs. (C) Mucin secretion from transverse colon. Blue indicates mucin stained with alcian blue at pH 2.5. Five to six rats per group were assayed in triplicate by H&E and alcian blue staining. Data represent the mean ± SD from three replicates. a, p < 0.05 compared to the No-treated group. b, p < 0.05 compared to the AEtLP-treated group. c, p < 0.05 compared to the Lop + vehicle-treated group.