Cell cycle phase analysis by flow cytometry in Huh7 cells treated with TD. The cells were incubated for 48 h with medium alone (control), 50 and 100 μg/ml of TD. A graphic representation of the concentration-dependent increase in the percentage of apoptotic cells following TD treatment. Results are expressed as a percentage of the sub-G1 population obtained from the cells of flow cytometry data of propidium iodide fluorescence as described in Materials and methods, mean ± SD (n =3). Statistical significance *P < 0.05 as compared to the control, in the absence of TD.