Figure 1

HPLC chromatograms of marker substances in individual herbs of Gami-Cheongyeul-Sodok-Eum (GCSE). HPLC analysis was performed on YMC-Pack Pro C18 column (4.6 × 250 mm, 5 μm). The optimum mobile phase and wavelength for detection were acetonitrile and water for (A) nodakenin, (B) decursin and (C) decursinol angelate (gradient, 20% - 80% ACN, 330 nm); (D) acetonitrile and water (3.4 g of monobasic potassium phosphate and 1.7 g of sodium lauryl sulfate/1000 mL) for berberine (1:1, 345 nm); aqueous acetic acid and acetonitrile for (E) glycyrrhizin (3:2, 254 nm) and (F) liquiritigenin (7.5:2.5, 276 nm); aqueous acetic acid and acetonitrile : methanol (7:3) for (G) baicalin, (H) baicalein, and (I) wogonin (gradient, 25% - 52% ACN-MeOH, 277 nm). Data are representative of three independent experiments.