Expressions of p35 and p25 were alternated after spinal ischemia-reperfusion injury. p25 was up-regulated (A), p35 was down-regulated (B), and Western blot was performed for analysis of expressions of p35 and p25 (C) after spinal ischemia-reperfusion injury. Administration of BYHWD and roscovitine influenced the alteration of both p25 and p35 expressions. *: P < 0.05, SI + BYHWD vs. SI + Saline group. *: P < 0.05, SI + R vs. SI + Saline group. ** P < 0.01, SI + Saline vs. Control group. SI + Saline: Spinal ischemia + saline. SI + BYHWD: Spinal ischemia + BYHWD. SI + R: Spinal ischemia + Roscovitine.