POCU1b inhibits adipocyte differentiation and GPDH activity. (A) Oil red O staining for lipid content in 3 T3-L1 adipocytes. 3 T3-L1 preadipocytes were induced to differentiate in POCU1b (5–25 μg/mL) for 12 days. (B) Relative density of Oil red O staining. Data are expressed as the mean ± S.E.M. (n = 5). **p < 0.01, and ***p < 0.001 vs. untreated cells, respectively. (C) Confluent 3 T3-L1 preadipocytes were induced to differentiate in the presence or absence of POCU1b (5, 15, or 25 μg/mL). Cells were lysed on day 12, and GPDH activity was measured. Data are expressed as means ± S.E.M. (n = 5) for three independent experiments, normalized to GPDH activity obtained under standard differentiation conditions. ***p < 0.001 vs. untreated cells.