Effect of β-sitosterol (ST) on apoptosis and Bax/Bcl-2 ratio in breast carcinoma cells. MDA-MB-231 cells were treated with DMSO (control) or 60 and 90 μM ST for 48 h. (A) Western blot analyses were performed to examine the expression level of Bax and Bcl-2 proteins. Membranes were probed with anti-Bax and Bcl-2 antibodies followed by peroxidase-conjugated appropriate secondary antibodies, and visualized by ECL detection system. Membrane was striped and re-probed with anti-β actin for loading control. Densitometric value of bands are shown below each band as fold change from that of control and corrected with loading control (B) Represents the ratio of Bax/Bcl-2 protein expression levels.