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Table 1 Primers used for RT-PCR analysis (F: forward, R: reverse)

From: Entada africana fraction CH2Cl2/MEOH 5% inhibits inducible nitric oxide synthase and pro-inflammatory cytokines gene expression induced by lipopolysaccharide in microglia

Gene Primer sequences
TNFα F 5′-TTGACCTCAGCGCTGAGTTG-3′
  R 5′-CCTGTAGCCCACGTCGTAGC-3′
IL-1β F5′-CAGGATGAGGACATGAGGCACC-3′
  R 5′-CTCTGCAGACTCAAACTCCAC-3′
IL6 F5′-GTACTCCAGAAGACCAGAGG-3′
  R 5′-TGCTGGTGACAACCACGGCC-3′
iNOS F5′-CCCTTCCGAAGTTTCTGGCAGCAGC-3′
  R5′-GGCTGTCAGAGCCTCGTGGCTTTGG-3
β-actin F5′-CCGTCTTCCCCTCCATCGT-3′
  R5′-ATCGTCCCAGTTGGTTACAATGC-3′