Primary rat ASMC. A: Cells growing around the tissues with spindle-shaped, cell body and central oval nuclei in 2–3 days, then cells confluence occurring in 8-10 days (phase-contrast microscopy). Bar = 100 μm. B: Typical “hill and valley” appearance in ASMC (HE staining). Bar = 50 μm. C: Positive α-actin fluorescent immunostaining with green parallel fiber in cytoplasm. Bar = 10 μm.