Effects of GTPs on the protein expression levels of claudin-5, occludin, ZO-1, and PKCα in ischemic cerebral microvessels after cerebral ischemia were determined by western blot. (A) The lanes are the following groups: V0–V120, 0, 30, 60, and 120 min after ischemia in the vehicle group; G0–G120, 0, 30, 60, and 120 min after ischemia in the GTPs-treated group. β-Actin served as a internal control. IDVs of claudin-5 (B), occludin (C), ZO-1 (D) and PKCα (E) are expressed as a fold reduction or a fold increase relative to the vehicle group of ischemia at 0 min. Data represent the means ± SD (n = 6, each). ** P < 0.01 vs. vehicle group of ischemia 0 min. #
P < 0.05, ##
P < 0.01 vs. vehicle group at the same time points.