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Table 2 ELISA Granzyme B, TNF-α and IFN-γ detection of untreated, human rIL-2 treated, direct or indirect R. korthalsii methanol extract stimulated Human NK cells

From: Rhaphidophora korthalsii modulates peripheral blood natural killer cell proliferation, cytokine secretion and cytotoxicity

  Granzyme B TNF-α IFN-γ
  (pg/mL)
CD56 NK cell    
Untreated NK cells 72.3 ± 9.5i 89.1 ± 11.3x 857.5 ± 19.5a
Human rIL-2 treated NK cells 86.2 ± 4.1i 743.3 ± 15.6y 3672.6 ± 28.1b
Direct R. korthalsii extract treated NK cells 79.1 ± 8.5i 478.9 ± 17.8z 2954.2 ± 27.9c
Indirect R. korthalsii extract treated NK cells 88.5 ± 7.3i 814.1 ± 21.3zz 6238.8 ± 38.2d
Direct R. korthalsii extract treated NK cells + MQ1-17H12 antibody 78.4 ± 5.7i 442.9 ± 26.5z 2713.9 ± 33.5c
Indirect R. korthalsii extract treated NK cells + MQ1-17H12 antibody 83.6 ± 6.2i 527.4 ± 31.3z 3112.2 ± 41.7c
CD3 T cell    
Untreated CD3 T cells 66.4 ± 4.7i 84.5 ± 9.3 x 691.2 ± 28.5e
Human rIL-2 treated CD3 T cells 69.8 ± 5.2i 876 ± 24.7zz 2976 ± 36.1c
Direct R. korthalsii extract treated CD3 T cells 65.3 ± 7.1i 215.8 ± 41.2zzz 977.2 ± 28.4f
Indirect R. korthalsii extract treated CD3 T cells 68.2 ± 8.3i 164.5 ± 33.9zzz 756.3 ± 23.9 e
  1. Values (n = 9; 3 independent experiments with three technical replicates each) represent the means ± S.E.M. Significant (P < 0.05). i indicate no significant different among the groups for Granzyme B; x, y, z, zz, zzz indicate significant different among the groups for TNF-α while a, b, c. d. e and f indicate significant different among the groups for IFN-γ (P < 0.05).