Chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of Cupressus lusitanica Mill. leaves from Cameroon

Table 3 Minimal inhibitory concentration (MIC)/Minimum bactericidal or fungicidal concentration (MBC or MFC) of test substances

Microoganisms	MIC		MBC/MFC
Gram (−) bacteria	Essential oil (%)	Reference (μg/ml)	Essential oil (%)	Reference^a(μg/ml)
Escherichia coli ATCC 11775	2.5	31.25	2.5	31.25
Klebsiella pneumoniae ATCC 13883	5.00	31.25	>10.00	31.25
Proteus mirabilis	1.25	125	1.25	250
Pseudomonas aeruginosa ATCC 27853	10.00	31.25	>10.00	31.25
Salmonella typhi ATCC 6539	2.5	62.50	2.5	125
Shigella flexneri	2.5	62.50	2.5	125
Gram (+) bacteria
Enterococcus faecalis ATCC 10541	1.25	15.62	2.5	15.62
Staphylococcus aureus ATCC 25922	1.25	62.50	2.5	62.50
Fungi
Candida albicans ATCC 9002	0.16	2	0.16	2
Candida glabrata CIPA 35	1.25	16	1.25	16
Candida krusei ATCC 6258	1.25	4	1.25	4
Candida lusitaniae ATCC 200950	0.62	2	0.62	>2
Candida parapsilosis ATCC 22019	1.25	16	1.25	16
Candida tropicalis ATCC 750	1.25	8	1.25	8

The results are the mean values of triplicate tests measured after 24 hrs (at 37°C for bacteria) and 48 hrs (at 35°C for fungi) incubation; ^a Reference compounds were gentamicin and nystatine for bacteria and fungi respectively.

ISSN: 2662-7671