Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand

Table 4 The percentage scavenging activity of the crude DCM extract of bee pollen (CDEb) and its subfractions (CDEb1 - CDEb7) following silica gel 60 quick column chromatography

Fraction	Percentage scavenging activity					EC₅₀	r ²
	10 µg/ml	50 µg/ml	100 µg/ml	400 µg/ml	1000 µg/ml	(µg/ml)
CDEb1	3.76 ± 4.27	0.04 ± 2.05	0.31 ± 2.54	0.00	8.29 ± 2.41	>1000
CDEb2	3.16 ± 1.47	0.00	0.00	0.00	5.92 ± 1.85	>1000
CDEb3	1.43 ± 2.07	0.1 ± 1.58	0.00	0.00	9.05 ± 0.54	>1000
CDEb4	0.00	0.00	0.00	14.35 ± 5.91	12.0 ± 0.54	>1000
CDEb5	5.15 ± 0.7	14.0 ± 3.04	22.5 ± 1.86	59.8 ± 3.98	65.3 ± 0.8	322 ± 27	1
CDEb6	3.69 ± 3.7	19.4 ± 3.80	37.6 ± 1.33	76.7 ± 1.2	78.4 ± 1.44	196 ± 9.9	1
CDEb7	3.34 ± 1.33	6.19 ± 2.13	9.69 ± 0.8	38.3 ± 4.8	58.5 ± 1.71	762 ± 67	1
CDEb	66.9 ± 0.88	68.8 ± 0.47	70.8 ± 0.73	70.1 ± 0.47	61.8 ± 0.7	7.47 ± 0.12	1
Ascorbic acid	58.4 ± 1.7	76.9 ± 1.3	76.8 ± 1.05	76.1 ± 0.72	65.2 ± 6.5	8.57 ± 0.24	1

The reference standard, ascorbic acid, is shown for comparison.
EC₅₀ values were calculated by linear regression (r ² represents the correlation coefficient). ND represents no available data. Data are shown as the means ± 1 SD and are derived from 3 independent repeats.

ISSN: 2662-7671