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Table 4 The percentage scavenging activity of the crude DCM extract of bee pollen (CDEb) and its subfractions (CDEb1 - CDEb7) following silica gel 60 quick column chromatography

From: Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand

Fraction Percentage scavenging activity EC50 r 2
  10 µg/ml 50 µg/ml 100 µg/ml 400 µg/ml 1000 µg/ml (µg/ml)  
CDEb1 3.76 ± 4.27 0.04 ± 2.05 0.31 ± 2.54 0.00 8.29 ± 2.41 >1000  
CDEb2 3.16 ± 1.47 0.00 0.00 0.00 5.92 ± 1.85 >1000  
CDEb3 1.43 ± 2.07 0.1 ± 1.58 0.00 0.00 9.05 ± 0.54 >1000  
CDEb4 0.00 0.00 0.00 14.35 ± 5.91 12.0 ± 0.54 >1000  
CDEb5 5.15 ± 0.7 14.0 ± 3.04 22.5 ± 1.86 59.8 ± 3.98 65.3 ± 0.8 322 ± 27 1
CDEb6 3.69 ± 3.7 19.4 ± 3.80 37.6 ± 1.33 76.7 ± 1.2 78.4 ± 1.44 196 ± 9.9 1
CDEb7 3.34 ± 1.33 6.19 ± 2.13 9.69 ± 0.8 38.3 ± 4.8 58.5 ± 1.71 762 ± 67 1
CDEb 66.9 ± 0.88 68.8 ± 0.47 70.8 ± 0.73 70.1 ± 0.47 61.8 ± 0.7 7.47 ± 0.12 1
Ascorbic acid 58.4 ± 1.7 76.9 ± 1.3 76.8 ± 1.05 76.1 ± 0.72 65.2 ± 6.5 8.57 ± 0.24 1
  1. The reference standard, ascorbic acid, is shown for comparison.
  2. EC50 values were calculated by linear regression (r 2 represents the correlation coefficient). ND represents no available data. Data are shown as the means ± 1 SD and are derived from 3 independent repeats.