Effect of SAP Aa D and furosemide on diuresis in rats. Animals were given an oral loading of physiological solution (0.9 % NaCl, 4 ml/100 g body weight) to impose a uniform water and salt state. In A: Rats were orally pretreated (30 min) with 0.5 ml 0.9 % NaCl and received the physiological solution alone (NaCl + NaCl) or containing 13 mg/kg furosemide (NaCl + Furo); SAPAaD + NaCl, rats were orally pretreated with 50 mg/kg SAPAa D and received the physiological solution alone. In B: Rats were orally pretreated with SAPAa D and received the physiological solution containing furosemide (SAPAaD + Furo); NaCl + AVP, rats were orally pretreated with 0.5 ml 0.9 % NaCl and received the physiological solution containing AVP. NaCl + Furo is the same as in A. Animals (n = 5/group) were individually housed in metabolic cages, and urine volume was measured every 30 min throughout the experiment (3 h). Data are expressed as means ± SEM. In A, *p < 0.05 vs. group NaCl + NaCl. In B,*p <0.05, **p < 0.01 and ***p < 0.001 vs. group NaCl + Furo at the correspondent time. Data were analyzed by two-way ANOVA followed by Bonferroni’s test.