Figure 8

Western blot analysis of phosphorylated-p53 and p21 proteins in cancer cells treated with P. indica leaf or root aqueous extract. (A) Western blot of p21 and phosphorylated-p53 proteins of GBM8401 cells after P. indica extract treatment. Lane 1: untreated; lane 2: 3 days of 0.1 mg/ml P. indica root aqueous extract treatment; lane 3: 3 days of 1 mg/ml P. indica root aqueous extract treatment; lane 4: 5 days of 1 mg/ml P. indica root aqueous extract treatment; lane 5: 3 days of 0.1 mg/ml P. indica leaf aqueous extract treatment; lane 6: 3 days of 1 mg/ml P. indica leaf aqueous extract treatment. (B) Western blot of p21, phosphorylated-p53, AKT, and phosphorylated-AKT proteins of HeLa cells after P. indica extract treatment. Lane 1: untreated; lane 2: 2 days of 0.25 mg/ml P. indica root aqueous extract treatment; lane 3: 2 days of 0.5 mg/ml P. indica root aqueous extract treatment. Each experiment was done in triplicate.