Effects of PF on histological changes. A, Sirius Red staining, ×100 magnification; B, desmin staining, brown staining indicates immunopositivity ×200 magnification, n=3; C, Hyp content; The number in Hyp and sirius red detection was the same as the number of animals in each group, data as mean ±SD, ☆
p<0.05 versus 2-week DMN rats; ⋆
p<0.05 versus DMN-water rats. Kruskal-Wallis test was used for group comparisons in Hyp. D, α-SMA and Col1(α1) using real-time PCR analysis, as an internal control, 18S rRNA was amplified (n=3), data as mean ±SD, ▴p<0.05 versus DMN-water rats; Δ
p<0.05 versus 2-week DMN rats. Bonferroni’s multiple comparison test were used for group comparisons in α-SMA and Col1(α1). E, the expression of desmin and Col1(α1) was analyzed using western blot in rat livers with DMN-induced liver fibrosis (n=3); western blotting of total protein extracts with antibodies recognizing desmin, Col1(α1), and GAPDH respectively.