Immunohistochemistry and Western blot analysis. Immunohistochemical analysis analysis of expression of Bax [A] and Bcl-2 [B] protein in HepG2 cells (shown by arrows). [a] control (untreated) [b] treated with the decoction (600 μg/ml), [c] treated with the decoction (1200 μg/ml). Section [d] represents the negative control. Hematoxyline was used for counterstaining. [C] Western blot analysis of effects of the decoction on Bax and Bcl-2 expression in HepG2 cells treated with different concentrations (lane 1-control, lane 2-300 μg/ml, lane 3-600 μg/ml, lane 4-1200 μg/ml) of the standardized decoction at (a) 12 h post incubation, (b) 24 h post-incubation and (c) 48 h post incubation was performed as described in the Materials and Methods section. Normalization of bands was performed using β-actin protein.