Figure 5From: 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations Significantly transient synergistic relationships between ACA and CDDP, suggesting that ACA plays a role in HSC-4 chemo-sensitization. (A) Isobologram IC50 analysis of HSC-4 cells after exposure to both ACA/CDDP simultaneous and sequential combinations over 48 h. C1: 24 h simultaneous treatment (constant ACA); C2: 24 h simultaneous treatment (constant CDDP); C3: 48 h simultaneous treatment (constant ACA); C4: 48 h simultaneous treatment (constant) CDDP; C5: 12 h pre-ACA prior to 24 h of CDDP sequential treatment; C6: 12 h pre-ACA prior to 48 h of CDDP sequential treatment. (B) Summary of IC50 and combination index (CI) values calculated from in vitro MTT cytotoxicity assays on various treatment combinations. Data were presented as mean ± SEM of independent triplicate experiments.Back to article page