Effect of ARA extract on lipid accumulation in 3T3-L1 cells. The cells were induced to differentiate into adipocytes with MDI medium with or without ARA extract (100, 200 and 500 mg/mL) and troglitazone (10 μg/mL) for 8 days. Troglitazone used as a positive control. (A) The cells were stained with Oil Red O on day 8. Representative photomicrographics (X 100) are shown for each treatment group. (B) To quantify lipid accumulation in the cells, Oil Red O dye was dissolved in isopropanol and the optical density was measured at 490 nm. Data was based on the OD values. Each value represents the mean ± S.D. (n = 3). *p < 0.05 vs. control. P: preadipocyte, C: control, 100: ARA 100 μg/mL, 250: ARA 250 μg/mL, 500: ARA 500 μg/mL and T: troglitazone 10 μg/mL.