Flow chart of extraction and column chromatographic separation of Bryophyllum pinnata leaves. 20 kg fresh leaves of B. pinnata were crushed with water and filtered. Residue was dried and extracted with chloroform and fractionated with gradient of petroleum ether and ethyl acetated followed by washing with methanol. Fractions were designated as F1 to F6 as indicated in the figure. Pet Eth, Petroleum Ether; Eth Acet, Ethyl Acetate; Meth, Methanol.