Effects of guggulsterone (GS) on the H9C2 cell line. (A) Cells were treated with various concentrations of GS for 24 h. Cell viability was determined using an MTT assay, as described in the Materials and Methods section. (B) GS cytotoxicity was analyzed using LDH release. The results are expressed as mean ± S.E. from 3 independent experiments. Asterisks indicate a significant difference for the control (GS 0 μM) (**P < 0.01).