Figure 4

Effect of HLM ( Uncaria tomentosa ., Boswellia spp ., Lepidium meyenii and L-Leucine ) on the IL-1β-induced gene expression of ACAN and COL2A1 in IL-1β-stimulated OA chondrocytes. Chondrocytes were pretreated for 2 h with HLM (1 and 5 μg/ml) and then stimulated or not stimulated with IL-1β (5 ng/ml) for 24 h, expression of ACAN (A) and COL2A1 (B) was analyzed by Real time-PCR. Relative gene expression was normalized to GAPDH and compared with un-stimulated control. Value represents Mean ± SD of three different experiments run in duplicate. * p < 0.05.