Figure 3

Effect of HLM ( Uncaria tomentosa ., Boswellia spp ., Lepidium meyenii and L-Leucine ) on the IL-1β-induced expression of MMP-9 and MMP-13 in IL-1β-stimulated OA chondrocytes. Chondrocytes were pretreated for 2 h with HLM (1-10 μg/ml) and then stimulated or not stimulated with IL-1β (5 ng/ml) for 6 h for MMP-9 mRNA (A) and 24 h for MMP-13 mRNA(B). mRNA expression was analyzed by Real time-PCR. Relative gene expression was normalized to GAPDH and compared with un-stimulated control. Levels of MMP-9 (C) and MMP-13 (D) in culture supernatants were quantified by sandwich ELISA at 24 h. Value represents Mean ± SD of three different experiments runs in duplicate. * p < 0.05.