Figure 2

Effects of L.obtusiloba extract on the basal and IGF-1-induced IGF-1R signal transduction. As described for Table 2, HCC cells treated for 24 h with 100 μg/ml L.obtusiloba extract, 125 ng/ml IGF-1, a combination of both or untreated cells were lysed. Whole cell lysates were analyzed by western-blot with antibodies specific for phosphorylated and normal IGF-1R and its downstream targets Akt and Stat3. Bands were visualized by chemiluminescence. Blots are representative for three independent experiments. Quantification of the protein-activation (n-fold to control values) were taken from table 3.