Effect of bitter melon juice on cellular lipid droplets. Total cellular lipid contents were significantly reduced in preadipocytes undergoing differentiation when treated with varying concentrations of BMJ (Figures 2A - 2C). Light microscopic pictures of oil red 'O' staining in untreated control adipocytes (A), and preadipocytes treated with - 0.5% BMJ (B), 1% BMJ (C) or 2% BMJ (D), during differentiation (40× magnification). Figure 1E depicts degree of preadipocyte differentiation during treatment as measured by total amount of extracted oil "O" red. Values represent the mean ± SE (n = 6) of three independent experiments conducted in duplicate. a, b, c, dMean values with common letters do not differ (p < 0.05).