Figure 3From: Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study Induction of apoptosis in COLO 320 DM. Human colon cancer cell lines (COLO 320 DM) cells were treated 120 or 240 μM β-sitosterol. The anti-proliferative effect is shown in (a), flow cytometric analysis for the determination of pro-apoptotic/apoptotic/necrotic cells is given in (b), and fluorescent staining of nuclei by Hoechst 33258 at 20× is seen in (c).Back to article page