Neuroprotective potentials of selected natural edible oils using enzyme inhibitory, kinetic and simulation approaches

Background Edible oils have proven health benefits in the prevention and treatment of various disorders since the establishment of human era. This study was aimed to appraise neuropharmacological studies on the commonly used edible oils including Cinnamomum verum (CV), Zingiber officinale (ZO) and Cuminum cyminum (CC). Methods The oils were analyzed via GC-MS for identifications of bioactive compounds. Anti-radicals capacity of the oils were evaluated via 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals scavenging assays. The samples were also tested against two important acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) which are among the important drug targets in Alzheimer’s disease. Lineweaver-Burk plots were constructed for enzyme inhibition studies which correspond to velocity of enzymes (Vmax) against the reciprocal of substrate concentration (Km) in the presence of test samples and control drugs following Michaelis-Menten kinetics. Docking studies on AChE target were also carried out using Molecular Operating Environment (MOE 2016.0802) software. Results (Gas chromatography-mass spectrometry GC-MS) analysis revealed the presence of thirty-four compounds in Cinnamon oil (Cv.Eo), fourteen in ginger oil (Zo.Eo) and fifty-six in cumin oil (Cc.Eo). In the antioxidant assays, Cv.Eo, Zo.Eo and Cc.Eo exhibited IC50 values of 85, 121, 280 μg/ml sequentially against DPPH radicals. Whereas, in ABTS assay, Cv.Eo, Zo.Eo and Cc.Eo showed considerable anti-radicals potentials with IC50 values of 93, 77 and 271 μg/ml respectively. Furthermore, Cv.Eo was highly active against AChE enzyme with IC50 of 21 μg/ml. Zo.Eo and Cc.Eo exhibited considerable inhibitory activities against AChE with IC50 values of 88 and 198 μg/ml respectively. In BChE assay, Cv.Eo, Zo.Eo and Cc.Eo exhibited IC50 values of 106, 101 and 37 μg/ml respectively. Our results revealed that these oils possess considerable antioxidant and cholinesterase inhibitory potentials. As functional foods these oils can be effective remedy for the prevention and management of neurological disorders including AD. Synergistic effect of all the identified compounds was determined via binding energy values computed through docking simulations. Binding orientations showed that all the compounds interact with amino acid residues present in the peripheral anionic site (PAS) and catalytic anionic site (CAS) amino acid residues, oxyanion hole and acyl pocket via π-π stacking interactions and hydrogen bond interactions. Supplementary Information The online version contains supplementary material available at 10.1186/s12906-021-03420-0.


Background
Functional food is a term used for the processed food ingredients that exhibit or regulates various physiological functions beside nutritional values. The word was introduced in Japan in the mid-1980s and use of functional foods is regulated by Japanese Ministry of Health and Welfare under the name Foods for Specified Health Use (FOSHU) [1]. More than hundred food products have been recognized by FOSHU, yet this term is relatively new in other countries. According to reports, the market value of functional foods is about 28.9 billion US dollars, though it greatly varies is different countries [2]. But it believed that functional foods would play a significant role in the prevention and treatment of diseases and promotion of health at affordable cost. The role of natural products-based diets in the prevention and treatment of diseases is evident from various studies [3,4]. A study revealed that the risk of cancer development is less among fruits and vegetables consumers when compared with non-users [5]. It is now well understood that secondary metabolites in these functional foods are responsible for various pharmacological properties including prevention of diseases and other pathological targets [5,6].
In the modern era, numerous health problems are known to be cured by modulation of nutritional habits and use of edible natural products in the diet [7,8]. Natural products have been used in various types of food items for therapeutic effects and preservation [9][10][11]. Among these the nutrition, essential oils and a variety of different plants extracts, and their spices have been a huge attention owing to their safety and efficacy profiles [12][13][14]. Literature showed that variety of herbs, spices and their essential oils get from medicinal plants are thoroughly employed for the treatment of neurodegenerative diseases, growing and survival of neuronal cell, physical and mental performance on experimental bases in which most of them are scientifically proved [15,16]. Cinnamon as well as cumin has a verity of antimicrobial, and antioxidants components [17]. Cumin and their chemical constituents use as anti-inflammatory and analgesic agents [18]. Cinnamon extract has been found to inhibit tau aggregation, an important hallmark of Alzheimer's disease (AD) [19]. Ginger used as food ingredient, is effective in inflammation [20], cough, asthma, muscle pain, bleeding, nausea [21]. Ginger in addition to other therapies has been recommended for brain disorders including paralysis via ischemic stroke [22]. Crude extract of ginger were previously reported to modulate brain cholinesterase's and reduce amyloid load in brain tissues [23].
Alzheimer's disease (AD) is a progressive neurodegenerative disorder which is characterized by cognitive dysfunction, behavioral turbulence and imperfection in activities of daily life [24]. The cognitive defect in AD is due to deterioration of cholinergic neurons particularly at the basal forebrain leading to impairment in neurotransmission in cerebral cortex and other brain parts [25]. An important target in AD is the use of cholinesterase inhibitors to overcome deficiency of a vital neurotransmitter acetylcholine (ACh) [26][27][28]. Use of free radicals scavengers are also indicated in several diseases including AD [29,30]. In AD patients Aβ cause liberation of excessive free radicals thus causing neurodegeneration and leading to chronic diseases like AD [31]. Antioxidants attenuate inflammation pathway by scavenging free radicals and ROS. So, the antioxidants may be useful in the protection from neurodegeneration in AD [32,33]. This study was designed to assess the neuroprotective role of three important edible oils in the context of their enzyme inhibitory and antioxidant potentials.

Oil analysis
Fresh rhizome of ginger and seeds of cumin and cinnamon were purchased from the market at Chakdara, Pakistan and subjected to hydro-distillation using a Clevenger apparatus coupled with condenser [34]. Distillation was continued at 100°C till enough oils were collected. Thereafter, anhydrous sodium sulfate was used for removal of traces of water and were refrigerated at -30°C till further use.

GC-MS analysis
For the GC-MS analyses ion trap MS spectrometer and a DB-1 MS fused silica nonpolar capillary column (30 m length, with 0.25 mm internal diameter, and 0.25 μm film thickness) was used with helium as carrier gas. The oven temperature was held for 5 min at inter scan delay. Identification and quantification of the essential oils components was performed on the basis of GC retention indices and computer matching with the NIST-2005, Wiley 275 and TRLIB Library, further uncertain identification was made by the comparison of the fragmentation patterns of mass spectra with previously reported in the literature [35,36].

DPPH anti-radicals study
The DPPH free radicals scavenging capacity of our oils was analyzed following standard protocol [37]. Briefly, a 0.004% methanolic solution of DPPH (200 μL) was mixed with equal volume of previously prepared samples solutions (1000, 500, 250, 125, 62.5, 31.25 μg/ml). The resultant mixture was maintained at room temperature for about 30 min. Pure methanol and Ascorbic acid solutions were used as negative and reference agents respectively. Absorbance's were measured at 517 nm via UV spectrophotometer and radicals scavenging was calculated as;

ABTS anti-radicals study
The anti-radicals potentials of selected oils was further evaluated via another in-vitro assay following our reported protocol [38]. In brief, 100 ml of 7 mmol ABTS methanolic solution was added to 100 ml of 2.45 mmol KH 2 PO 4 solution followed by addition of 100 ml water. The resultant mixture as overnight incubated at dark to generate sufficient free radicals. Subsequently, ABTS solution absorbance was adjusted to 0.7 at 745 nm via addition of 50% methanolic solution. Thereafter, 3ml of ABTS solution was added to 3 ml of samples in UV covet and absorbance was recorded via UV spectrophotometer. Ascorbic acid was used as control drug in the study. ABTS scavenging activity was calculated as;

Cholinesterase inhibition assays
Anti-cholinesterase potentials of selected essentials oils were evaluated by some modification of the spectrophotometric analysis respectively followed by [39]. Different dilutions of plant fraction were made ranging from (1000 to 62.5 μg/ml). Briefly, 10 μl each of acetylcholinesterase and butyrylcholinesterase 0.03 U/ml solution were mixed with 410 μL of different essential oils samples taken in a cuvette. Subsequently, DTNB solution (10 μL) was transferred to the mixture solution, mixed well and incubated at 30°C for 15 min. To start enzymatic reaction, 10μL substrates were added to the reaction mixture. Absorbance's were recorded at 412 nm via microplate reader. Percent inhibition of AChE and BChE were evaluated by judgment of rates of reaction of tests samples comparative to that of control. Reaction mixture containing all substances without tested sample was used as control. All experiments were repeated three times. Galantamine, the anti-cholinesterase kind of drug was used as standard. The percent inhibition and percent enzymatic potential were calculated as follows;

Determination of IC 50
From inhibition values IC 50 were determined using their dose-response curves [40].

Estimation of kinetic parameters
The Kinetic parameters were estimated via construction of Lineweaver-Burk plots (1/v versus 1/[s]) where v is apparent velocity reaction and [s] at the concentrations used for substrates and enzymes. The V max and K m values were determined via Michaelis Menten kinetics [41].

Docking studies
Identified compounds were docked against the target site of enzymes using Molecular Operating Environment (MOE 2016.0802) suite [41,42]. Briefly, crystal structure of Torpedo California AChE (TcAChE, PDB code 1EVE) with native ligand donepezil was acquired from Protein Data Bank. Whereas, legands preparation, identification of enzymes active sites as well as detailed docking method was adopted from our previously reported procedure [43,44]. Likewise, three-dimensional interaction plot of the drug-enzyme complex was analyzed by using Discovery Studio Visualizer (Dassault Systems BIOVIA 2017) [45].

Statistical analysis
Data were expressed as mean ± S.E.M. Multiple group means of parametric data sets were compared using twoway analysis of variance (ANOVA) after it was determined that the data conformed to a normal distribution. If an overall significance was found, a multiple-comparisons test was used using GraphPad Prism 5 (GraphPad Software Inc. San Diego CA, USA) [46]. A value of P < 0.05 was considered as significant.

Phytochemistry of oils
GC-MS analysis of the selected oils revealed the presence of potent secondary metabolites. We identified thirtyfour (34) compounds in the oils obtained from cinnamon as shown in Fig. 1  details for the identified compounds are provided in Table  S1 of the supporting information. The GCMS analysis of Zingiber officinale oil confirm the presence of lesser number of compounds (Compounds ZO1 -14, Fig. 2). Total of fourteen potent compounds were identified. The chemical names, retention times, molecular formula and other details of the identified compounds are shown in Table  S2 of the supporting information. The oils obtained from Cuminum cyminum showed relatively larger number of bioactive phytochemicals. In C. cyminum oil, total of fiftysix compounds were identified by GCMS analysis. The structures of compounds (CC1 -56) are shown in Fig. 3 while the chemical names, retention times with the specified method and other details are provided in Table S3 of the supporting information file.

Antioxidant studies
The three edible oils, i.e., cinnamon, ginger and cumin were tested for their antioxidant assays using DPPH (Fig. 4) and ABTS (Fig. 5) free radicals scavenging assays. All the three oils were tested at concentrations of 62.50-1000 μg/ ml in comparison to the positive control, ascorbic acid in both DPPH and ABTS assays as shown in Table S4 of the supporting information. The three edible oils have strong DPPH free radicals scavenging tendency and in comparison to the positive control, ascorbic acid, these oils showed a significant difference in their percentage inhibitions at the tested concentrations of 62.50-1000 μg/ml (P < 0.001).
In the DPPH assay, the calculated values of IC 50 were 85, 121 and 280 μg/ml for cinnamon, ginger and cumin oils respectively, while for the positive control, ascorbic acid it was observed as 06 μg/ml. Similarly, in the ABTS assay, the three edible oils showed marked antioxidant propensity as observed from the percentage inhibition of ABTS free radicals, which were found to be significantly different at the tested concentrations of 62.5 μg/ml (P < 0.01 for cinnamon and ginger, P < 0.001 for cumin), and 125-1000 μg/ml (P < 0.001) as compared to the positive control, ascorbic

Docking studies against acetylcholinesterase (AChE) target
Binding orientations, interactions and energy data has a key role to explore the possible mechanism of inhibition. In case of AChE inhibition, interactions with the peripheral anionic site (PAS) and catalytic anionic site (CAS) amino acid residues are important for further chemical modification and structure activity relationship exploration. In case of plant extracts, the role of each constituent is also important to determine the synergistic effect. Hence, to further strengthen our study, we carried out docking simulation using MOE software. We docked all the identified compounds from oils of cinnamon, ginger, and Cumin into the binding site of Torpedo California AChE (TcAChE, PDB code 1EVE). Three-dimensional (3-D) interaction plots of important compounds with binding energy value greater than -7.00 kcal/mol are shown in Figs. 8, 9 and 10. All the identified compounds from cinnamon are shown in pink stick form. While compounds from ginger and cumin are shown in turquoise and orange color, respectively. Compounds containing aromatic rings forms π-π stacking interactions with catalytic anionic site residue Trp84, Phe330 and Phe331. Peripheral anionic site (PAS) residues Tyr121 and Tyr334 also formed π-π stacking interactions with the aromatic rings. Hydroxyl, and carbonyl oxygen groups forms hydrogen bond interactions with amino acid residues present in the active of AChE. Compounds with aliphatic chains showed binding energy values between 4.0 and 5.5 kcal/mol.

Discussions
Free radicals generated during metabolic processes are involved in the progression of numerous diseases including AD. The human immune system antioxidant enzymes normally neutralize these free radicals and thus protect us against the hazardous actions of these radicals [48,49]. However, under unusual circumstance when production of these free radicals is excessive or human immune system is compromised, then these free radicals exhibit diverse degenerative effects [50,51]. Subsequently, exogenous supplementation of antioxidants is extremely necessary to counteract the unwanted effects of these free radicals. Currently beside new drugs discovery, use of functional foods is gaining attention. These substances beside their nutritional values significantly contribute towards the prevention and treatment of various diseases. The essential oils used in the current study has a wide use in foods and are common spices. Subsequently, they were tested for their in vitro scavenging potentials against free radicals and inhibitory activities against vital enzymes implicated in AD. In the current study, we employed two extensively used antioxidant methods including DPPH and ABTS assays [52,53]. In DPPH radical scavenging assay, cinnamon, ginger, cumin and standard exhibited IC 50 values of 85, 121, 280 and 06 μg/ml respectively. The IC 50 value of cinnamon oil was very comparative to the standard drug ascorbic acid (Fig. 4). These results suggest that cinnamon essential oil has high radical scavenging activity. Further, ABTS assays was also used to evaluate the antioxidant potentials of selected samples. When ABTS radical is mixed with an antioxidant agent, ABTS radical accept electron and is converted to a non-radical form. Color change indicates reduction of the ABTS radical [54]. Cinnamon, ginger and cumin oils showed considerable ABTS radicals scavenging activity. The IC 50 values of cinnamon, ginger and cumin and standard drug were 93, 77, 271 and 08 respectively (Fig. 5). The details about DPPH and ABTS assays are provided in Table S4 of the supporting information.
Cholinesterase's are important enzymes responsible for removal of ACh from the synaptic cleft. The enzyme metabolizes acetylcholine after their interaction with cholinergic receptors and is recycled [55]. As there is deficiency of ACh in AD patients, so an important therapeutic option is to use inhibitors of cholinesterase's so that any acetylcholine available at synapses may remain for prolong time and symptoms related to acetylcholine deficiency will be relived [56]. Based on the idea, few cholinesterase inhibitors were developed and approved for clinical use. Among these, one drug (galantamine) is from natural sources whereas, rivastigmine is synthetic derivative of a naturally isolated compounds. However, these agents have limited efficacy and some side effects. So, there is dire need for the discovery and development of more useful cholinesterase potentially from natural sources. In the current study, our test sample exhibited considerable cholinesterase inhibitory potentials. The IC 50 values of cinnamon, ginger, cumin and standard drug against acetyl cholinesterase were 21, 88, 198 and 08 μg/ml respectively. Whereas the IC 50 values of cinnamon, ginger, cumin standard against BChE enzyme were 106, 101, 37 and 06 μg/ml respectively (Table 1).

Conclusions
In the present study cinnamon, ginger and cumin exhibited considerable anti-radical and cholinesterase inhibitory potentials. GCMS revealed the presence of several bioactive compounds which might be implicated in the pharmacological properties of essential oils. Being commonly used spices, these agents might be useful for the prevention of memory-related degenerative disorders like AD. However, furthermore detailed animal studies are required regarding the molecular mechanism and potential use of these agents for the said properties. We docked all the identified compounds from oils of cinnamon, ginger, and Cumin into the binding site of enzyme.