Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components

Background Pyrola decorata H. Andres, is exclusively distributed in China and a source of traditional Chinese herbal medicine Luxiancao for more than 2000 years. Here, we evaluated the antioxidant and cytoprotective effects of P. decorata and its five phenolic components (protocatechuic acid, gallic acid, hyperoside, 2′′-O-galloylhyperin, and quercetin), and discussed their antioxidant chemistry. Methods A lyophilized aqueous extract of P. decorata (LAEP) was prepared and analyzed with high-performance liquid chromatography (HPLC). LAEP and its five phenolic components were comparatively investigated using five antioxidant assays, including ferric-reducing antioxidant power, cupric ion-reducing antioxidant capacity, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO•)-scavenging, 1,1-diphenyl-2-picryl-hydrazl radical (DPPH•)-scavenging, and 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) radical (ABTS+•)-scavenging activities. The reaction products of the five phenolic components with 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxyl radical (4-methoxy-TEMPO•) were determined with ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) analysis. LAEP and its five phenolic components were incubated with bone marrow-derived mesenchymal stem cells (bmMSCs) subjected to oxidative stress to demonstrate their cytoprotective effects with a flow cytometry assay. Results In the five antioxidant assays, LAEP and its five phenolic components dose-dependently increased the radical-scavenging (or reducing power) activities. However, the IC50 values of hyperoside were consistently higher than those of 2′′-O-galloylhyperin. UPLC-ESI-Q-TOF-MS/MS analysis results indicated that the five phenolics could yield dimer products in the presence of 4-methoxy-TEMPO• via the radical adduct formation (RAF) pathway. Flow cytometry assay results confirmed the cytoprotective activity of LAEP and its five phenolic components toward stressed bmMSCs. In particular, 2′′-O-galloylhyperin could more effectively reduce the percentage of damaged bmMSCs than hyperoside. Conclusion LAEP and its five phenolic components may undergo redox-based pathways (such as electron transfer and H+ transfer) and covalent-based pathway (i.e., RAF) to exhibit antioxidant activity. One consequence of RAF is the generation of phenolic-phenolic dimer. In both organic and aqueous media, 2′′-O-galloylhyperin exhibited higher redox-based antioxidant levels (or cytoprotective levels) than those with hyperoside. The differences could be attributed to 2′′-O-galloylation reaction.

The dried whole herb of P. decorata can act as the resource of Chinese herbal medicine Luxianao [7]. Luxianao however has been used in traditional Chinese medicine (TCM) for more than 2000 years. Modern pharmacological investigation has suggested that Luxiancao (or its bioactive components) exhibit anti-osteoarthritis [8], antiinflammatory [8], hepatoprotective [9], and anti-influenza [10] properties. These pharmacological effects are known to be closely associated with its antioxidant and cytoprotective actions [4,[11][12][13]. However, the antioxidant mechanisms and cytoprotective effects of Luxiancao (P. decorata) have not been reported yet, regardless that its extract has been evaluated for the antioxidant capacity using chemical approach [4].
To investigate the antioxidant mechanisms, the study tried to firstly analyze the antioxidant phenolics of P. decorata using HPLC technology. These phenolics were further measured for the radical adduct formation (RAF) possibility using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-offlight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/ MS) technology. Especially, two flavonoids hyperoside (i.e., hyperin) and 2′′-O-galloylhyperin were comparatively analyzed in the study, this is because that: (1) they have been demonstrated to co-exist in P. decorata [4,14,15]; (2) Essentially, 2′′-O-galloylhyperin is a galloylation ester of hyperoside at 2′′-O-position ( Fig. 1). Thereby, the comparison between them can be used to analyze the antioxidant structure-activity relationship of 2″-Ogalloylation modification.
To explore the cytoprotective effects of P. decorata, bone marrow-derived mesenchymal stem cells (bmMSCs) were isolated from bone marrow of femur and tibia of rats [16,17]. bmMSCs are known to possess characteristics of stem cells, including self-renewal and multidirectional differentiation properties [18], and bmMSC transplantation has been applied to treat heart diseases [19], spinal cord injuries [20] and diabetes [21]. During the process of diffusion and differentiation, chemical or physical factors, such as radiation and iron overload, could produce reactive oxygen species (ROS), resulting in the oxidative stress-induced damage to bmMSCs [22,23]. The oxidative damage however has limited bmMSCs in transplantation engineering application. The study tried to use flow cytometric assay to evaluate the possible cytoprotective effect of P. decorata on oxidative stressed bmMSCs.
Apparently, the evaluation of the cytoprotective effects of this plant may improve our understanding of the beneficial properties of Luxiancao, and provide important information on its phenolics in MSCs transplantation engineering. The conclusion of the antioxidant structureactivity relationship of hyperoside and 2′′-O-galloylhyperin can also be used to explain the antioxidant difference between myricetin-3-O-rhamnopyranoside and myricetin-3-(2′′-O-galloyl)-O-rhamnopyranoside [24].

Reagents and materials
Protocatechuic acid (C 7  . P. decorata was subjected to extraction based on the guidance of the manufacturer (Fig. 2). In brief, the newly purchased P. decorata was decocted twice using 50-fold distilled water at 100°C for 5 min. The extract was concentrated then lyophilized at − 60°C under vacuum (1 Pa) for 24 h to obtain a lyophilized aqueous extract of P. decorata (LAEP). LAEP was stored at 5°C until further use.

Metal-reducing assays
The metal-reducing assays included ferric-reducing antioxidant power (FRAP) and cupric ion (Cu 2+ )-reducing antioxidant capacity (CUPRAC). The FRAP assay was carried out as per the method of Benzie and Strain [25]. In brief, the FRAP reagent was freshly prepared by mixing 10 mM TPTZ, 20 mM FeCl 3 , and 0.25 M acetate buffer (pH 3.6) at a ratio of 1:1:10. LAEP (5 mg/mL, x = 2-10 μL) or its five phenolic components (0.25 mg/mL, x = 2-10 μL) were added to (20 − x) μL of methanol and treated with 80 μL of FRAP reagent. After incubation for 30 min, the absorbance of the mixture was measured at 593 nm wavelength (A 593nm ) using acetate buffer as the blank on a microplate reader (Multiskan FC, Thermo Scientific, Shanghai, China). The relative reducing antioxidant power of the sample as compared to the maximum absorbance was calculated by the following formula: Where A min is the lowest A 593nm value in the experiment, A is the A 593nm value of the reaction mixture with sample, and A max is the greatest A 593nm value in the experiment.
The CUPRAC assay was conducted as per the method of Apak [26]. In brief, 12 μL CuSO 4 aqueous solution (10 mM), 12 μL neocuproine methanol solution (7.5 mM), and (75 − x) μL CH 3 COONH 4 buffer solution (100 mM, pH 7.4) were added to test tubes and incubated with different volumes of samples (10 mg/mL for LAEP or 0.5 mg/mL for the five phenolic components, x = 2-10 μL). The total volume was adjusted to 100 μL with buffer and the reactants were vigorously mixed. Absorbance against a buffer blank was measured at 450 nm wavelength using the microplate reader after 30 min. The relative reducing power of the sample as compared with the maximum absorbance was calculated using the formula presented for FRAP assay, wherein A min is the lowest A 450nm value in the experiment, A is the A 450nm value of the reaction mixture with sample, and A max is the greatest A 450nm value in the experiment.
The ABTS +• -scavenging activity was evaluated according to the previous method [28]. ABTS +• was produced by mixing 0.2 mL of ABTS diammonium salt (7.4 mmol/L) with 0.2 mL of potassium persulfate (2.6 mmol/L). The mixture was incubated in the dark at room temperature (27°C) for 12 h to complete the radical generation process before dilution with distilled water (at a ratio of approximately 1:20); the absorbance at 734 nm was 0.35 ± 0.01. To determine the scavenging activity, the x μL sample (x = 2-10 μL, 1 mg/mL for LAEP or 0.05 mg/mL for the five phenolic components) was added to (20 − x) μL of distilled water and treated with 80 μL of ABTS +• reagent. The absorbance at 734 nm was measured 3 min after initial mixing using distilled water as the blank. The percentage of ABTS +• -scavenging activity was calculated using the formula presented for PTIO • -scavenging assay, wherein A 0 is the absorbance at 734 nm of the control (reaction system without sample) and A is the absorbance at 734 nm of the reaction mixture with the sample.
The DPPH • radical-scavenging activity was determined as previously described [29]. Briefly, 80 μL of DPPH •methanolic solution (0.1 mol/L) was mixed with x μL sample-methanolic solution (for LAEP: x = 1-5 μL, the concentration was 2.5 mg/mL; for the five phenolics: x = 4-20 μL, the concentration was 0.01 mg/mL) and (20x) μL methanol. The mixture was maintained at room temperature for 30 min, and the absorbance was measured at 519 nm on microplate reader against a buffer blank. The percentage of DPPH • -scavenging activity was calculated based on the formula presented for PTIO • -scavenging assay, wherein A 0 is the absorbance at 519 nm of the reaction system without sample and A is the absorbance at 519 nm of the reaction mixture with sample.
Ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC−ESI − Q − TOF − MS/MS) analysis UPLC−ESI − Q − TOF − MS/MS analysis of reaction products of 4-methoxy-TEMPO • with the five phenolic components was conducted as per the method described in our previous report [30]. The methanolic solutions of phenolic components were mixed with a solution of 4methoxy-TEMPO • in methanol at a molar ratio of 1:2, and the resulting mixtures were incubated for 24 h at room temperature. The product mixtures were filtered through a 0.22-μm filter and analyzed with a UPLC-ESI-Q-TOF-MS/MS system equipped with a C 18 column (2.0 mm i.d. × 100 mm, 2.2 μm, Shimadzu Co., Kyoto, Japan). The mobile phase used for elution comprised a mixture of acetonitrile (phase A) and 0.1% formic acid in water (phase B). The column was eluted at a flow rate of 0.2 mL/min with the following gradient elution program: 0-2 min, maintained at 30% B; 2-10 min, 30-0% B; and 10-12 min, 0-30% B. The sample injection volume was set at 5 μL, while the column temperature was 35°C.
The Q-TOF-MS/MS analysis was conducted on a Triple TOF 5600 plus mass spectrometer (AB SCIEX, Framingham, MA, USA) equipped with an ESI source in the negative ionization mode. The scan range was set at 100-2000 Da. The system was run with the following parameters: ion spray voltage, − 4500 V; ion source heater, 550°C; curtain gas (CUR, N 2 ), 30 psi; nebulizing gas (GS1, air), 50 psi; Tis gas (GS2, air), 50 psi. The declustering potential (DP) was set at − 100 V, whereas the collision energy (CE) was set at − 40 V at a collision energy spread (CES) of 20 V. The RAF products were quantified by extracting corresponding formula (e.g., [C 14

Protective effect on bmMSCs against • OH-induced oxidative stress (flow cytometric assay)
Eight male Sprague-Dawley rats (4 weeks old, 60-80 g) were obtained from the Experimental Animal Center of Guangzhou University of Chinese Medicine (Guangzhou, China) and housed in there at 25 ± 2°C, and exposed to a 12 h/12 h light-dark cycle with free access to food and water. Then, four Sprague-Dawley mice were euthanized by cervical dislocation to obtain enough bone marrowderived mesenchymal stem cells (bmMSCs) from the bone marrow of femur and tibia of the rats.
The bmMSCs were cultured as previously described with some modifications [31]. Briefly, the cells were collected by gradient centrifugation at 900×g for 30 min on 1.073 g/mL Percoll. The prepared cells were detached by treatment with 0.25% trypsin and transferred into culture flasks at a density of 1 × 10 4 cells/cm 2 . The cells were evaluated for homogeneity through the detection of CD44 expression via cytometry, and seeded at 1 × 10 6 cells/well into 12-well plates. After adherence for 24 h, bmMSCs were divided into control, model, and sample groups. In the control group, bmMSCs were incubated for 24 h in DMEM, while the cells from the model and sample groups were incubated in the presence of FeCl 2 (100 μM) and then treated with by H 2 O 2 (50 μM). The cells from the sample group were incubated with 3 μg/mL of sample.
After incubation for 10 min, the mixture of FeCl 2 and H 2 O 2 was removed. The cells were washed twice with 0.1 mol/L cold phosphate-buffered saline (PBS) and resuspended in a binding buffer at a concentration of 1 × 10 6 cells/mL. About 100 μL of the solution (1 × 10 5 cells) was transferred to a 5 mL culture tube and treated with 5 μL of fluorescein isothiocyanate (FITC)-Annexin V and 5 μL PI. The cells were vortexed and incubated for 15 min in the dark and treated with 400 μL of binding buffer, followed by analysis with flow cytometry (Accuri C6, Franklin Lakes, BD, USA) with standard software.

Statistical analysis
Each experiment was performed in triplicates; the data were recorded as mean ± standard deviation (SD). The doseresponse curves were plotted using Origin 2017 professional software (OriginLab, Northampton, MA, USA). The IC 50 value was defined as the final concentration of 50% radical inhibition (or relative reducing power). Statistical comparisons were carried out with one-way analysis of variance (ANOVA) to detect significant differences using SPSS 13.0 software (SPSS Inc., Chicago, IL, USA) for Windows. A value of p < 0.05 was considered statistically significant.

Preparation and HPLC analysis of LAEP
The appearance of LAEP was a brown powder (Additional file 7), and its yield was calculated as 6.87%. The prepared LAEP was analyzed with HPLC and found to exhibit at least five different phenolic components, including protocatechuic acid, gallic acid, hyperoside, 2′ ′-O-galloylhyperin, and quercetin at 0.55, 0.31, 0.16, 0.12, and 0.08%, respectively. The retention times of these compounds were 5.369 min, 8.168 min, 18.062 min, 18.663 min, and 27.670 min, respectively (Fig. 3).

Metal-reducing assays
In the study, two metal-reducing assays (i.e., FRAP assay and CUPRAC assay) were carried out to demonstrate the reducing power of antioxidants. As illustrated in Additional file 8, in FRAP assay, LAEP and its five phenolic components respectively increased the FRAP percentages in a dose-dependent manner. According to the IC 50 values in Table 1, their Fe 3+ -reducing levels roughly increased in the order of protocatechuic acid < quercetin < gallic acid < Fig. 3 HPLC analysis of LAEP. LAEP means the lyophilized aqueous extract of P. decorata. The determining wavelength was 280 nm; the longitudinal axis was the strength of absorbance; mAu, milli-absorbance unit; Rt, retention time; Min, minute hyperoside < 2"-O-galloylhyperin. In CUPRAC assay, LAEP and its five phenolic components respectively increased the CUPRAC percentages in a dose-dependent manner, and their order of Cu 2+ -reducing levels increased in the order of protocatechuic acid < gallic acid < quercetin < hyperoside < 2"-O-galloylhyperin (Table 1).

Free radical-scavenging assays in vitro
As shown in Additional file 8, LAEP and its five phenolic components could successfully scavenge PTIO • at pH 4.5 and pH 7.4. However, according to the IC 50 values in Table 1, their relative PTIO • -scavenging levels at the same pH value were different from each other. Also, each of the five phenolic components exhibited different PTIO • -scavenging levels between pH 4.5 and pH 7.4.
The DPPH • -scavenging and ABTS +• -scavenging assays have been widely used in antioxidant studies. As seen in Additional file 8, LAEP and its five phenolic components concentration-dependently increased the DPPH •scavenging and ABTS +• -scavenging percentages. The IC 50 values were detailed in Table 1.
Protective effect on bmMSCs against • OH-induced oxidative stress (flow cytometric assay) As shown in Fig. 5, both LAEP and its five phenolic components had significantly (p < 0.05) decreased the percentages of damaged cells compared with the model group. In particular, the percentage of damaged cells was significantly (p < 0.05) lower in 2′′-O-galloylhyperin-treated group than in hyperoside-treated group.

Discussion
In the study, five phenolic components have been found in P. decorata, including two phenolic acids (protocatechuic acid and gallic acid), and three flavonoid glucosides (hyperoside, 2′′-O-galloylhyperin, and quercetin) (Fig. 3). The detection of two phenolic acids undoubtedly has added new information of phytochemistry of P. decorata species [14,15]. Regardless that the five phenolic components have also been expressed in P. incarnata; however, their contents in LAEP were slightly lower than those in P. incarnata [5,32,33]. The difference could be attributed to the species difference, the extraction processes employed. Furthermore, harvest seasons may also influence the contents of phenolics in Pyrola L. plants [33].
The five phenolic components and LAEP thus were further estimated using various antioxidant assays, such as FRAP and CUPRAC assays. The FRAP and CUPRAC assays are based on the electron transfer (ET) reaction at pH 3.6 and 7.4, respectively [34,35]. Hence, the concentration-dependent increase of LAEP and its five phenolic components in reducing powers suggests that they could be involved in the ET pathway to exert antioxidant effects at pH 3.6 and 7.4.
To investigate whether the ET pathway could mediate radical-scavenging process, LAEP and its five phenolic components were further evaluated with PTIO • -scavenging assay. The PTIO • radical is present as a stable form in aqueous buffers at various pH values, and its scavenging reaction at pH 4.5 was confirmed as a pure ET pathway by cyclic voltammetry [36]. The results demonstrated that LAEP and its five phenolic components could exhibit elevated PTIO •scavenging activities at pH 4.5, indicative of their abilities to undergo ET reaction to scavenge radicals. However, at pH 7.4, LAEP and its five phenolic components could also effectively scavenge PTIO • radical and their IC 50 values at The IC 50 value was defined as the final concentration corresponding to 50% radical inhibition (or relative reducing power) obtained from the dose-response curves in Additional file 8, as analyzed by Origin 2017 professional software (OriginLab, Northampton, MA, USA). The IC 50 values of the five phenolics with different superscripts ( a , b , c , or d ) in the same row are significantly different (p < 0.05). Trolox ((±)-6-hydroxyl-2,5,7,8-tetramethlychromane-2-carboxylic acid) served as the positive control. LAEP means the lyophilized aqueous extract of P. decorata. Each experiment was performed in triplicates; the data were recorded as mean ± standard deviation (SD) pH 7.4 were lower than those at pH 4.5 (Table 1). These pH-mediated effects suggest the involvement of the H + transfer pathway in the PTIO • -scavenging process [27]. Besides aqueous buffers, aqueous solution was used as the experimental medium to evaluate the antioxidant properties in the ABTS +• -scavenging assay. Furthermore, an organic medium was used in the DPPH • -scavenging assay. The effectiveness of LAEP and its five phenolic components in the two assays revealed their ability to exert antioxidant actions in both organic and aqueous media.
The five detected phenolic components had different IC 50 values in each antioxidant assay (Table 1). In particular, hyperoside showed higher IC 50 values than 2′′-O-galloylhyperin in each of antioxidant assay, suggesting that 2′ ′-O-galloylhyperin had higher antioxidant effects based on ET and H + transfer pathways than hyperoside. As previously discussed, the sole structural difference between hyperoside and 2′′-O-galloylhyperin is the presence of 2′ ′-O-galloyl moiety. Hence, the difference in their antioxidant activities could only be attributed to the role of galloylation. The pyrogallol moiety in 2′′-O-galloyl provides additional hydroxyl groups with radical-scavenging and metal-chelating abilities (Fig. 6) [24,[37][38][39][40].  Both ET and H + transfer pathways are associated with the redox-based antioxidant mechanisms [41][42][43]. Through redox-based antioxidant mechanisms, new radical intermediates may be generated [30,44]. These radical intermediates (including radical) may covalently link with each other to form a radical adduct. This is called as the radical adduct formation (RAF) mechanism. Dimerization however is one type of RAF. As seen in Fig. 4, protocatechuic acid, gallic acid, hyperoside, 2′′-O-galloylhyperin, and quercetin respectively produced the m/z values 306, 338, 926, 1230, and 602. These m/z values are exactly two units lower than twice their molecular weights of (M.W. 154, 170, 464, 616, and 302). We assumed that the two phenolic molecules were dimerized via one covalent bond. The observation of dimer formation indicates the presence of RAF mechanism in the antioxidant process associated with the five phenolic components in LAEP.
Of these phenolic components, protocatechuic acid belongs to the phenolic acid family, whereas 2′′-O-galloylhyperin is a member of the flavonoid family. According to the MS/MS data, the dimeric products of protocatechuic acid and 2′′-O-galloylhyperin could be fully elucidated, as shown in Fig. 7. These elucidations further supported the presence of RAF mechanism. Furthermore, these elucidations clearly suggest that one consequence of RAF is the generation of phenolic-phenolic dimer. The dimer with covalent linkage, however, could effectively diminish the free radical to terminate the radical chain reaction [11].
The high resolution MS spectra provided an accurate m/z value for various fragments. For instance, the loss from m/z 151.0044 to 107.0152 was exactly the m/z value of CO 2 . The experimental m/z value of CO 2 was 43.9892 (151.0044-107.0152) (Fig. 4a2 and Fig. 7a), while the calculated m/z value was 43.9899. The relative bias was only 1.6 × 10 − 5 . In addition, the m/z value from 151.0044 to 123.0099 was equal to 28. This loss was considered to be from N 2 or CO. However, the accurate m/z value was 27.9945, indicating that the loss was related to CO (calculated m/z value 27.9949) than to N 2 (calculated m/z value 28.0061) ( Fig. 4A2 and Fig. 7a). The MS elucidations shown in Fig. 7 suggest the possible presence of other linking sites between two phenolic moieties, and that other reasonable cleavages should not be excluded in the MS elucidation.
To investigate whether LAEP and its five phenolic components could resist the oxidative stress-induced damage in biological system, we incubated bmMSCs damaged with Fenton reagent (an • OH radical generator) in the presence of these compounds in an aqueous buffer. The percentage of damaged cells was characterized via flow cytometry. The results that LAEP and its five phenolic components could significantly (p < 0.05) decrease the percentages of damaged cells could be partly responsible for the pharmacological effect of Luxiancao. Furthermore, P. decorata and its phenolic components could function as therapeutic candidates for oxidative damages in bmMSC transplantation.
As mentioned above, the 2′′-O-galloylhyperin-treated group showed significantly (p < 0.05) higher cellular viability than hyperoside-treated group. Thus, 2′′-O-galloylhyperin could improve the cytoprotective effect of phenolic glycosides in aqueous buffer, consistent with the findings of antioxidant assays in organic and aqueous media. In bmMSC transplantation, galloylation of phenolic glycosides could be considered as a promising agent to improve the effectiveness of cytoprotectors.

Conclusion
P. decorata contains at least five phenolic components, including protocatechuic acid, gallic acid, hyperoside, 2′ ′-O-galloylhyperin, and quercetin. These compounds may undergo redox-based pathways (such as ET and H + transfer) and covalent-based pathway (i.e., RAF) to exhibit antioxidant activities. RAF could yield a stable phenolic-phenolic dimer. In comparison with hyperoside, 2′′-O-galloylhyperin showed better redox-based antioxidant activity (or cytoprotective activity) in both organic and aqueous media. The improvement stems from the 2′′-O-galloylation modification, which adds adjacent hydroxyl groups with radical-scavenging and metal-chelating abilities.