Fig. 5

Immunohistochemical analysis of GFAP in the cerebral cortex. Deconvoluted images of DAB staining (second row) was produced by IHC profiler, and the percentage contributions was calculated relative to each image pixel intensity as high positive, positive, low positive, and negative pixels. Automated score of low positive is found for the control group and LPS + OM group, while LPS group presented as positive. The generated automated score for OM group is negative. For further quantitative analysis, calculation of IHC optical density score was done. LPS group scored 2.154 compared with 1.583 for control group, 1.5626 for LPS + OM group, and 1.4937 for OM group. Our results show that GFAP expression was enhanced in the LPS model and was attenuated under the effect of OM extract (COX-2 immunostaining, × 80) (n = 3)