Fig. 4

Effects of PSC-FEs treatment on AKT and AMPK phosphorylation in insulin-resistant HepG2 cells. After induction of insulin-resistance, HepG2 cells were treated with low (5 ug/ml) and high (50 ug/ml) doses of total and methanolic PSC-FE for 24 h. The cells were lysed and subjected to western blot analysis for AKT, p-AKT, AMPK and p-AMPK. Representative western blot for phosphorylated and total AKT and AMPK are shown in (A). Quantitative analysis of p-AKT/AKT and p-AMPK/AMPK are shown in (B) and (C), respectively. Treatment with 1 mM metformin was considered as positive control. Equal Protein loading was ensured by β-actin. Data represent mean ± SD from three individual experiments performed in triplicate. ** p < 0.01 and *** p < 0.001 compared with untreated insulin-resistant cells (model). ### p < 0.001 compared with untreated HepG2 cells (control)