Fig. 3

Cantharidin treatment is involved in EZH2/H3K27me3 related cell cycle pathway regulation. (A) GO functional annotation shows that cantharidin treatment is positively associated with H3K27me3 pathway based on GSEA analysis. (B) The heatmap shows H3K27me3 related genes expression in cantharidin treated HepG2 cell. Red represents up-regulation genes; blue represents down-regulation genes. (C) The expression of PRC2 complex genes, the key regulatory enzyme of H3K27me3, in cantharidin treated HepG2 cell. The *P < 0.05 was regarded as statistically significant. (D) Venn diagram shows the target genes of cantharidin downregulation genes and H3K27me3 significantly enriched genes from ChIP-seq results in HepG2 cell. (E) The heatmap shows the expression of 97 genes from venn analysis, which may be target genes of H3K27me3 in cantharidin treatment. Red represents up-regulation genes, green represents down-regulation genes. (F) Go enrichment analysis of the predicted target gene set. (G) Pathway analysis of the predicted target gene set. (H) PPI network of these target genes based on the STRING database results