Fig. 6

Effects of baicalein on post-adsorption stage of ZIKV infection. A549 cells were infected with MOI 1 of ZIKV prior to treating infected cells with 100 µM baicalein at 0, 1, 3, 6, 9 h (0, + 1, +3, + 6, +9) after infection. Cells were incubated until 24 h post infection. Viral infectivity was determined by flow cytometry (A), viral productivity was determined by plaque assay (B), and viral genome copy number was determined by qRT-PCR (C). Viral titer and viral genome copy number are shown in a logarithmic scale. Error bars show mean ± SD and p-value compared to DMSO control of each time-point (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001). UT = untreated