Fig. 1

Cytotoxicity and EC₅₀ of baicalein and orlistat towards A549 cells. A, B A549 cells were treated with varying concentrations (1-800 µM) of baicalein for 24 h. Cell viability was determined by (A) the MTT assay and (B) a trypan blue exclusion assay. Experiments were undertaken independently in quadruplicate. C A549 cells were infected with ZIKV with MOI 1 and treated with varying concentrations (1-200 µM) of baicalein for 24 h. Viral titer was determined from cell culture supernatant by plaque assay to calculate the EC₅₀. D, E A549 cells were treated with varying concentrations (1-400 µM) of orlistat for 24 h. Cell viability was determined by (D) the MTT assay. Experiments were undertaken independently in quadruplicate. E A549 cells were infected with ZIKV with MOI 1 and treated with varying concentrations (1-400 µM) of orlistat for 24 h. Viral titer was determined from cell culture supernatant by plaque assay to calculate the EC₅₀. Experiments were undertaken in triplicate with duplicate plaque assay. Error bars show mean ± SEM and p-value compared to DMSO control (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001)