Fig. 7

The main compounds of KJG (GRg1 and Ssd) reduced neuroinflammation response by inhibiting TLR4 via PI3K/AKT/FOXO1 pathway in LPS-induced BV2 cells. (a) The levels of TNF-α, IL-6, and IL-1β in BV2 cells and their statistical analysis (n = 3). (b) WB analysis for TLR4, p-PI3K, PI3K, p-AKT, AKT, p-FOXO1, and FOXO1in BV2 cells (n = 3). (c) WB analysis for FOXO1 in nucleus and cytoplasm of BV2 cells (n = 3). One-way ANOVA: # P < 0.05, ## P < 0.01, ### P < 0.001 vs. blank group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. LPS group. & P < 0.05, && P < 0.01, &&& P < 0.001 vs. LPS + LY + GRg1 group or LPS + LY + Ssd group