Fig. 3

KJG ameliorates depressive behavior by reducing neuroinflammation in CUMS-induced mice. (a) The effect of KJG on the depressive behaviors induced by CUMS in mice (5 weeks, n = 10). The sucrose preference test (SPT, first), the open field test (OFT, second), the Tail suspension test (TST, third), and the forced swimming test (FST, fourth). (b) The morphological changes of CA1 region in hippocampal tissue (n = 3). Nissl staining (the left), and immunofluorescence staining of TLR4 and their statistical analysis (the right) (Red, TLR4; blue, DAPI; Scale bar: 50 μm). (c) The levels of TNF-α, IL-6, and IL-1β in the serum of mice and their statistical analysis (n = 8). One-way ANOVA: # P < 0.05, ## P < 0.01, ### P < 0.001 vs. control group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. CUMS group; & P < 0.05, && P < 0.01, &&& P < 0.001, vs. CUMS + KJG8 group. KJG4, 4000 mg crude drug/kg; KJG8, 8000 mg crude drug/kg