Fig. 3
From: Cytotoxic activity of essential oil from Leaves of Myrcia splendens against A549 Lung Cancer cells
Morphological changes in the cytoskeleton and nucleus of A549 cells, observed with DAPI and Phalloidin/FITC staining, after treatment for 24 h with M. splendens EO at concentrations of 10, 20 and 40 µg/mL. Doxorubicin (25 µg/mL) was used as a positive control. Filled arrows represent chromatin condensation and DNA fragmentation; hollow arrows represent reduction in cytoplasmic volume and thin arrows, rounding and cell shrinkage. Bar: 20 μm