Fig. 2

Antibacterial components in the TEOs distilled from fresh plant material after TLC-DB associated with chromatographic separation. Mobile phase: toluene-ethyl acetate 95:5 (v/v); 1 µL indicated the applied volumes of the EOs and 0.2 µL for the standard. (A) TLC plate under UV 254 nm, (B) TLC plate after treatment with vanillin-sulfuric acid reagent and documented in visible light, (C) TLC-DB assay: Bioautograms using H. influenzae, (D) TLC-DB assay: Bioautograms using H. parainfluenzae, (E) TLC-DB assay: Bioautograms using P. aeruginosa (bright zones indicate antibacterial effects); T1-TEO distilled from fresh plant material and collected at the beginning of flowering period (200 mg/mL), T2-TEO distilled from fresh plant material and collected in full bloom (200 mg/mL), T3-TEO distilled from fresh plant material and collected at the end of flowering period (200 mg/mL), thym.-thymol standard (20 mg/mL)