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Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Correction: Protocatechuic aldehyde acts synergistically with dacarbazine to augment DNA double-strand breaks and promote apoptosis in cutaneous melanoma cells

Fig. 3

PA promotes MGMT degradation in melanoma cells. A-B Left panel shows western blot analysis for MGMT levels in A375 and SK-MEL-28 cells after 72h of treatment with DMSO, PA, DTIC or combination of PA and DTIC. Right panel represents the quantification of the MGMT protein levels for the left panel. C MGMT mRNA expression in A375 and SK-MEL-28 after 72 h PA treatment. D-E A375 and SK-MEL-28 were treated with cycloheximide in the presence or absence PA for 0-8 h hours. Western blot showed the MGMT degradation rates. Right panel represents quantification of the MGMT protein levels. F-G A375 and SK-MEL-28 were treated with MG132 combined with cycloheximide in the presence or absence of PA for 0-8 h hours. The MGMT protein levels were analyzed by western blot. Right panel represents quantification of the MGMT protein levels. ns = no significant, *p < 0.05, **p < 0.01,***p < 0.001 and **** p<0.0001

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