Fig. 5

Effects of EDAC on astrocytes in raised glutamate in hippocampal slices. Hippocampal slices from P10-12 GFAP-EGFP mice were treated for 24 h with high glutamate and different concentrations of EDAC, or DMSO vehicle in controls. A Representative confocal images of the different treatment groups (as indicated) illustrating changes in GFAP-EGFP + astrocytes (green); cell nuclei are labelled with Hoechst (blue). Scale bar 50 µm. B GFAP-EGFP relative fluorescence intensity was measured in constant FOV in the CA1 strata oriens, pyramidale and radiatum. Results were expressed as a % of the control and plotted as mean ± SEM (n = 4 for each treatment group). C GFAP-EGFP positive cells counting. Counts were performed of total SOX10-EGFP + cells in both strata oriens, pyramidale and radiatum. Data were tested for significance using one-way ANOVA, followed by Tukey’s post-hoc tests; **p < 0.01 and ***p < 0.0001 indicate comparisons with control, and #p < 0.05, ##p < 0.01 and ###p < 0.001 indicate comparisons with 3 mM glutamate