Fig. 3

Effects of EDAC on glutamate transporter GLAST expression in hippocampal organotypic cultures. Hippocampal slices from P10-12 mice were maintained in organotypic culture for 13 DIV, then treated for 24 h with 3 mM glutamate with or without EDAC (1 or 10 μg/mL) and then immunolabeled for GLAST, controls did not receive glutamate. A Representative confocal images from the different experimental groups, as indicated, immunofluorescence labeled for GLAST (white) and counterstained with Hoechst for cell nuclei (blue). B Representative confocal images from the different experimental groups, as indicated, immunofluorescence labelled for GLAST (white). Scale bar 20 μm. C Relative fluorescence intensity of GLAST immunostaining in the different layers of the CA1 (strata oriens, pyramidale and radiatum), as indicated, expressed as a % of control (DMSO), and plotted as mean ± SEM (n = 6 per experimental group). Data were tested for significance using one-way ANOVA, followed by Tukey’s post-hoc tests, # p < 0.05, represents a statistical difference compared to 3 mM glutamate