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Fig. 2 | BMC Complementary Medicine and Therapies

Fig. 2

From: Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia

Fig. 2

Effects of EDAC on glutamate transporter GLT1 expression in hippocampal organotypic cultures. Hippocampal slices from P10-12 mice were maintained in organotypic culture for 13 DIV, then treated for 24 h with 3 mM glutamate with or without EDAC (1 or 10 μg/mL) and then nuclei were stained with Hoechst 33,342 (A) and immunolabeled for GLT1 (B), controls did not receive glutamate. A Representative confocal images from the different experimental groups, as indicated, immunofluorescence labelled for GLT1 (white) and counterstained with Hoechst for cell nuclei (blue). C Representative confocal images from the different experimental groups, as indicated, immunofluorescence labelled for GLT1 (white). Scale bar 20 μm. C Confocal image of hippocampal slice (taken using × 5 objective) from the P6 GFAP-EGFP mouse shows the CA1 region and its layers labelled, strata oriens, pyramidale and radiatum analyzed, which the IF intensity was analyzed. In green, GFAP positive cells, in white NeuN to label neurons, and in blue nuclei stained with Hoechst.The Scale bar 200 µm. D Relative fluorescence intensity of GLT1 immunostaining in the different layers of the CA1, as indicated, expressed as a % of control (DMSO), and plotted as mean ± SEM (n = 6 per experimental group). Data were tested for significance using one-way ANOVA, followed by Tukey’s post-hoc tests, # p < 0.05, represents a statistical difference compared to 3 mM glutamate

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