Development of a novel, entirely herbal-based mouthwash effective against common oral bacteria and SARS-CoV-2

Table 3 Tested microorganisms and their role related to oral health

Name	Reference number	Type	Occurrence	Characteristics	Incubation conditions	Used media	Reference
Bacillus subtilis	SZMC 0209	Gram-positive	skin, oral flora, gastrointestinal tract	non-pathogen	30°C	Mueller–Hinton	[64, 65]
Escherichia coli	ATCC 25922	Gram-negative	oral flora, gastrointestinal tract	intestinal and extraintestinal pathogen	30°C	Mueller–Hinton	[66]
Lactobacillus plantarum	ATCC 10241	Gram-positive	oral flora	pH reducer	30°C	Rogosa	[6]
Pseudomonas aeruginosa	ATCC 27853	Gram-negative	oral flora	nosocomial pathogen	30°C	Mueller–Hinton	[67, 68]
Staphylococcus aureus	ATCC 29213	Gram-positive	oral flora	most common bacterial pathogen	30°C	Mueller–Hinton	[69]
Streptococcus mutans	ATCC 25175	Gram-positive	oral flora	pioneer bacteria of caries	30°C	Blood agar	[70]
Streptococcus sanguinis	ATCC 10556	Gram-positive	oral flora	pioneer bacteria of caries	30°C	Blood agar	[71]

ISSN: 2662-7671