Table 1 Primary antibodies used in western blot
antibody | species | company | dilution rate |
|---|---|---|---|
Cleaved caspase-3 | Rabbit monoclonal | CST 9661 | 1:1000 |
caspase-3 | Rabbit monoclonal | CST 14,220 | 1:1000 |
Cleaved-PARP | Rabbit monoclonal | CST 5625 | 1:1000 |
Bcl-2 | Mouse monoclonal | CST 17,447 | 1:1000 |
Bax | Rabbit monoclonal | CST 41,662 | 1:1000 |
β-actin | Mouse Monoclonal | Proteintech 60,008–1-Ig | 1:1000 |
E-cadherin | Rabbit monoclonal | CST 3195 | 1:1000 |
N-cadherin | Rabbit monoclonal | CST 13,116 | 1:1000 |
P-stat3 | Rabbit monoclonal | CST 9145 | 1:1000 |
Stat3 | Rabbit monoclonal | CST 12,640 | 1:1000 |
AKT | Rabbit monoclonal | CST 4685 | 1:1000 |
P-akt | Rabbit monoclonal | CST 4060 | 1:1000 |
C-myc | Rabbit monoclonal | CST 18,583 | 1:1000 |
Snail | Rabbit monoclonal | CST 3879 | 1:1000 |
p-mtor | Rabbit monoclonal | CST 5536 | 1:1000 |
JAK1 | Rabbit monoclonal | CST 29,261 | 1:1000 |
MMP2 | Rabbit polyclonal | Proteintech 10,373–2-AP | 1:1000 |
MMP9 | Rabbit polyclonal | Proteintech 10,375–2-AP | 1:1000 |
CyclinB1 | Rabbit monoclonal | CST 12,231 | 1:1000 |
CDK2 | Rabbit polyclonal | Proteintech 10,122–1-AP | 1:1000 |
CDC2 | Rabbit monoclonal | CST 28,439 | 1:1000 |
Secondary Antibodies | Goat Anti-Rabbit IgG | Proteintech SA00001-2 | 1:2000 |
Secondary Antibodies | Goat Anti-Mouse IgG | Proteintech SA00001-1 | 1:2000 |