Fig. 6

Osthole promotes apoptosis of bladder cancer cell and block cell cycle. A The apoptosis of bladder cancer detected by cell flow cytometry assays. B Bladder cancer cell cycle was detected by cell flow cytometry assays after treatment with Osthole. C The proportion of four cell staining states {AV(-) PI(-), AV( +) PI(-), AV( +) PI( +), AV(-),PI( +)} in bladder cancer cell. D The distribution of bladder cancer cell in different cell cycle. E The apoptosis related proteins (Bcl-2, Bax, Caspase3, Cleaved Caspase3, Cleaved parp) level were detected by western blot, β-actin was regard as control. F The G2 cell cycle related proteins (CyclinB1, CDC2) was detected by western blot. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the control group (0 μM)