Six Spain Thymus essential oils composition analysis and their in vitro and in silico study against Streptococcus mutans

Table 4 Antibacterial activity of different amounts of the Thymus essential oils

Essential oils	DIZ (mm)	Sensitivity index	MIC (μL/mL)	MBC (μL/mL)	MBC: MIC
TEO1	8.8 ± 0.20	+	-	-	-
TEO2	19.5 ± 1.00	+	-	-	-
TEO3	29.5 ± 2.60	+ +	-	-	-
TEO4	31.1 ± 0.20	+ + +	1.25	5.00	4:1
TEO5	33.4 ± 1.10	+ + +	0.625	1.25	2:1
TEO6	39.3 ± 0.70	+ + +	0.625	2.50	4:1
P/S	41.1 ± 2.20	+ + +	0.078125	0.625	8:1

Inhibition is expressed in mm and include the diameter of paper disc (6 mm). Results are shown as not sensitive (0) for a DIZ than 8 mm, moderately sensitive ( +) for 8—20 mm DIZ, sensitive (+ +) for 20—30 mm DIZ, and very sensitive (+ + +) for DIZ more than 30 mm; Minimal inhibitory concentration (MIC) and minimal bactericide concentration (MBC) (μL/mL) of TEO4, TEO5 and TEO6 on S. mutans ATCC700610. The TEOs were considered bactericidal when the MBC: MIC ratio was 2: 1, and bacteriostatic if this ratio was higher than 2: 1

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