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Fig. 2 | BMC Complementary Medicine and Therapies

Fig. 2

From: Effects of Leea indica leaf extracts and its phytoconstituents on natural killer cell-mediated cytotoxicity in human ovarian cancer

Fig. 2

Methyl gallate pre-treatment increased susceptibility of ovarian cancer cells to NK cell-mediated cytolysis. OVCAR-5 cells were pretreated overnight with or without (A) 0.03 mg/mL isolated gallic acid, (B) 0.1 mg/mL isolated methyl gallate, (C) 0.03 mg/mL gallic acid standard, (D) 0.1 mg/mL methyl gallate standard, (E) 20 µM oxaliplatin, and then co-cultured with activated NK cells at the various E:T ratios. Cytotoxicity assay was determined by measuring the viability of PKH-26 labelled target cancer cells on 96-well plate. Experiment was performed in triplicates. Results from one representative experiment of three are shown and presented as mean ± SD. Blue line represents non-treated cells, while red line represents treated cells. **p < 0.01; ***p < 0.001; ns, not significant

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